mouse anti il 17a mab (Sino Biological)
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mouse anti il 17a mab
Mouse Anti Il 17a Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+il+17a+mab/IL17+%2F+IL17a+Neutralizing+Antibody/pmc12639604-61-20-28
Average 93 stars, based on 3 article reviews
Mouse Anti Il 17a Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+il+17a+mab/IL17+%2F+IL17a+Neutralizing+Antibody/pmc12639604-61-20-28
Average 93 stars, based on 3 article reviews
mouse anti il 17a mab - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine"
Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine
Journal: Journal of Genetic Engineering & Biotechnology
doi: 10.1016/j.jgeb.2025.100613
Figure Legend Snippet: SDS-PAGE and Western blot analysis of hScFv-IL-17A proteins expressed in various E. coli strains. (a) SDS-PAGE of insoluble fractions; (b) SDS-PAGE of soluble fractions; (c) Western blot of insoluble fractions; (d) Western blot of soluble fractions. Lane 1: M (molecular weight marker); Lanes 2–4: hScFv-IL-17A-WT expressed in E. coli Origami B (DE3), SHuffle, and BL21 (DE3) with DisCoTune, respectively; Lanes 5–7: hScFv-IL-17A-C97S expressed in the same strains; Lane 8: purified hScFv M6-1B9 (positive control). Western blot detection was performed using an anti-His tag antibody. The arrow indicates the expected size of hScFv-IL-17A (∼28 kDa). Estimated amounts of hScFv-IL-17A are labeled. < LOD indicates signals below the limit of detection.
Techniques Used: SDS Page, Western Blot, Molecular Weight, Marker, Purification, Positive Control, Labeling
Figure Legend Snippet: The expression efficiency of soluble hScFv-IL-17A-WT (orange) and hScFv-IL-17A-C97S (green) in different expression systems: (WT/Ori and C97S/Ori) from Origami B (DE3); (WT/SHuffle and C97S/SHuffle) from SHuffle; and (WT/BL21Dc and C97S/BL21Dc) from BL21 (DE3) with DisCoTune. Data represent mean ± SD from three independent biological replicates. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Expressing
Figure Legend Snippet: Indirect ELISA of hScFv-IL-17A binding to hIL-17A using an avidin–biotin system. Purified hScFv-IL-17A-WT and hScFv-IL-17A-C97S were tested at 1 and 5 µg/mL, expressed from SHuffle (WT/SHuffle-1, WT/SHuffle-5; C97S/SHuffle-1, C97S/SHuffle-5; light/dark green) and BL21 (DE3) with DisCoTune (WT/BL21Dc-1, WT/BL21Dc-5; C97S/BL21Dc-1, C97S/BL21Dc-5; light/dark orange). An IL-17A mAb (dark grey) was used as a positive control. Data represent mean ± SD from three independent biological replicates. Statistical analysis was determined by unpaired two-tailed Student’s t -test. Significance levels are denoted as: ** p < 0.01, *** p < 0.001, **** p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Indirect ELISA, Binding Assay, Avidin-Biotin Assay, Purification, Positive Control, Two Tailed Test
Figure Legend Snippet: Binding assessment of hScFv-IL-17A-WT (a) and hScFv-IL-17A-C97S (b) from BL21 (DE3) with DisCoTune by competitive ELISA. Various concentrations (0.1, 1, and 2 µg/mL) of IL-17A mAb (neutralizing antibody) were tested in competition with hScFv-IL-17A. WT was used at 1 µg/mL, whereas C97S was used at 5 µg/mL to compensate for its reduced binding activity observed in indirect ELISA. An anti-IFN-γ mAb (clone B27) was used as an irrelevant antibody control. Data represent mean ± SD from three independent biological replicates.
Techniques Used: Binding Assay, Competitive ELISA, Activity Assay, Indirect ELISA, Control
Figure Legend Snippet: Structural alignment of hScFv-IL-17A-WT and hScFvIL-17A-C97S. The predicted structures were generated using ABodyBuilder2. The conformational structures of hScFv-IL-17A-WT and hScFv-IL-17A-C97S were compared using UCSF ChimeraX software. The red ribbon indicates hScFv-IL-17A-WT structure, and the gold ribbon indicates hScFv-IL-17A-C97S structure. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Generated, Software
Related Articles
Purification:Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFv-IL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFvIL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, Incubation:Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFv-IL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFvIL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, Positive Control:Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFv-IL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, Article Title: Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine Article Snippet: After washing, non-specific binding was blocked with a blocking solution (2 % Bovine serum albumin (BSA) in PBS with 0.05 % tween 20) for 1 h, and 50 μL of biotinylated-hIL-17A (1 μg/mL) (Sino Biological, Beijing, China) was added and incubated for 1 h at room temperature (RT). .. After washing, purified hScFv-IL-17A-WT and hScFvIL-17A-C97S (1 and 5 μg/mL) were added and incubated for 1 h. In this experiment, |
